Team:UNIPV-Pavia/Freezer

From 2010.igem.org

(Difference between revisions)
Line 37: Line 37:
<partinfo>BBa_K165018</partinfo>
<partinfo>BBa_K165018</partinfo>
</td><td>
</td><td>
-
ADH1 terminator, 253 bp
+
NLS-ADH1 terminator, 253 bp (Silver Standard 5' and 3')
</td><td>
</td><td>
08/06/2010
08/06/2010
Line 45: Line 45:
J63009 (Amp)
J63009 (Amp)
</td><td>
</td><td>
-
 
+
Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 64: Line 64:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
none
+
Seq ok(MIT)
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 82: Line 82:
pSB1A1
pSB1A1
</td><td>
</td><td>
-
none
+
Seq ok(MIT 2009); length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 99: Line 99:
pSB1A2
pSB1A2
</td><td>
</td><td>
-
none
+
Seq ok(MIT)
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 117: Line 117:
pSB1A2
pSB1A2
</td><td>
</td><td>
-
none
+
Seq ok(MIT); length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 126: Line 126:
<partinfo>BBa_K125500</partinfo>
<partinfo>BBa_K125500</partinfo>
</td><td>
</td><td>
-
GFP fusion brick, 718bp
+
GFP fusion brick, 718bp (Silver Standard 5', Knight Standard 3')
</td><td>
</td><td>
08/06/2010
08/06/2010
Line 134: Line 134:
pSB1A2
pSB1A2
</td><td>
</td><td>
-
none
+
Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
Line 152: Line 152:
pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
none
+
Wrong clone
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>
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pSB1AK3
pSB1AK3
</td><td>
</td><td>
-
none
+
length ok
</td>  
</td>  
<td></td><td></td>
<td></td><td></td>

Revision as of 09:40, 21 June 2010
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Freezer Management



Biobrick or
ligation name		DescriptionCreation dateStrainVectorQuality controlLengthStore

<partinfo>BBa_E2050</partinfo>

mOrange, 744bp

08/06/2010

E. coli DH5-alpha

pSB2K3

Seq ok(MIT); orange pellet when contained in pSB2K3

<partinfo>BBa_K165018</partinfo>

NLS-ADH1 terminator, 253 bp (Silver Standard 5' and 3')

08/06/2010

E. coli DH5-alpha

J63009 (Amp)

Seq partially confirmed(MIT) because it lacks a T at the 3', but the ADH1 terminator (i.e. <partinfo>BBa_K165010</partinfo>) is ok. The correct ApE file is here.

<partinfo>BBa_K165037</partinfo>

tef2 promoter, 403bp

08/06/2010

E. coli DH5-alpha

pSB1AK3

Seq ok(MIT)

<partinfo>BBa_P1004</partinfo>

Chloramphenicol resistence
cassette, 769 bp

08/06/2010

E. coli DH5-alpha

pSB1A1

Seq ok(MIT 2009); length ok

<partinfo>BBa_J61001</partinfo>

R6K origin, 406bp

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok(MIT)

<partinfo>BBa_K081008</partinfo>

RBS-luxI, 664bp

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq ok(MIT); length ok

<partinfo>BBa_K125500</partinfo>

GFP fusion brick, 718bp (Silver Standard 5', Knight Standard 3')

08/06/2010

E. coli DH5-alpha

pSB1A2

Seq partially confirmed(MIT) for unknown reasons: the alignment is fully correct. The ApE file with the correct prefix and suffix is here. Length ok.

I0-1

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

Wrong clone

I0-2

<partinfo>BBa_K125500</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

length ok

I1-1

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

J63009 (Amp)

none

I1-2

<partinfo>BBa_E2050</partinfo> (E-S)
+
<partinfo>BBa_K165018</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

J63009 (Amp)

none

I2-1

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

none

I2-2

<partinfo>BBa_P1004</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

none

I3-1

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

none

I3-1

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

none

I3-2

<partinfo>BBa_K081008</partinfo> (E-S)
+
<partinfo>BBa_B0015</partinfo> (E-X)

11/06/2010

E. coli DH5-alpha

pSB1AK3

none

I4-1

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

pSB1AK3

none

I4-2

<partinfo>BBa_K165037</partinfo> (S-P)
+
I1 (X-P)

18/06/2010

E. coli DH5-alpha

pSB1AK3

none

I5

I2 (E-S)
+
<partinfo>BBa_J61001</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A2

none

I6-2

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A3

none

I6-3

I3 (E-S)
+
<partinfo>BBa_T9002</partinfo> (E-X)

18/06/2010

E. coli DH5-alpha

pSB1A3

none

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