Team:MIT mge
From 2010.igem.org
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<div id="unique" style="padding:5px; font-size: 14px; border: 1px solid black; margin:5px;"> | <div id="unique" style="padding:5px; font-size: 14px; border: 1px solid black; margin:5px;"> | ||
- | <table width=70%><tr><td><div class="bodybaby">mammalian | + | <table width=70%><tr><td><div class="bodybaby">mammalian genetic engineering protocol</div></td> |
- | <tr><td><br>The Mammalian team | + | <tr><td><br>The Mammalian team created lines of cells using lentiviruses for transfection.<br><br> |
<div class="outline"> | <div class="outline"> | ||
<a href="#htd">1 HTD Preparation Protocol</a><br> | <a href="#htd">1 HTD Preparation Protocol</a><br> | ||
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</div></td><tr><td><br> | </div></td><tr><td><br> | ||
- | <div class="bodybaby" id="htd"> | + | <div class="bodybaby" id="htd">Calcium Phosphate Transfection</div><br> |
- | <b class="bolded" id="pour"> | + | <b class="bolded" id="pour">Beforehand:</b><br> |
<ul id="procedure"> | <ul id="procedure"> | ||
<li>Combine CaCL2 and dilute Na2PO4 to get CaPO4 with DNA in it</li> | <li>Combine CaCL2 and dilute Na2PO4 to get CaPO4 with DNA in it</li> | ||
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<ul><li>No cells (see precipitate, like fine snow on cells)</li><li>Just carrier DNA (any plasmid)</li></ul> | <ul><li>No cells (see precipitate, like fine snow on cells)</li><li>Just carrier DNA (any plasmid)</li></ul> | ||
<li></li> | <li></li> | ||
- | </ul> | + | </ul><br> |
- | <b class="bolded" id="pour"> | + | <b class="bolded" id="pour">Protocol</b><br> |
<ul id="procedure"> | <ul id="procedure"> | ||
<li>1. Mix DNA (different plasmids in right amounts) with CaCl2</li> | <li>1. Mix DNA (different plasmids in right amounts) with CaCl2</li> | ||
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</ul> | </ul> | ||
<br> | <br> | ||
- | < | + | <div class="bodybaby" id="htd">Filter and Concentrate Virus</div><br> |
+ | <b class="bolded" id="baking">Protocol</b><br> | ||
<ul id="procedure"> | <ul id="procedure"> | ||
<li>1. Start with 20 + 20 mL culture supernatant</li> | <li>1. Start with 20 + 20 mL culture supernatant</li> |
Revision as of 17:36, 16 October 2010
Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Protocol
Microfluidic stress
Mammalian Protocol
Microfluidics Protocol
Genetic Engineering Protocol
mammalian genetic engineering protocol |
The Mammalian team created lines of cells using lentiviruses for transfection.
1 HTD Preparation Protocol 1.1 PDMS Mixture Preparation 1.2 PDMS Pouring 1.3 PDMS Baking 1.4 PDMS-Device Punching and Bonding 1.5 PDMS Device Bonding 1.6 PDL coating 1.7 Collagen filling 1.8 Cell Seeding 2 Protocol for Deflection Experiments 2.1 Tubing Setup Details 2.2 Adding Medium to Channels 2.3 Connecting device to pressure valve 2.4 Microcontroller details |
Calcium Phosphate Transfection Beforehand:
Protocol
Filter and Concentrate Virus Protocol
|