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| - | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
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| - | <a href="https://static.igem.org/mediawiki/2010/e/ee/IC_Team22.jpg"><img src="https://static.igem.org/mediawiki/2010/e/ee/IC_Team22.jpg" width="200"/></a>
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| - | </html>
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| - | |Maddie
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| - | <a href="https://static.igem.org/mediawiki/2010/b/b2/IC_Team23.jpg"><img src="https://static.igem.org/mediawiki/2010/b/b2/IC_Team23.jpg" width="200"/></a>
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| - | </html>
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| - | |-
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| - | |Maddie
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| - | </div>
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| - | <a href="https://static.igem.org/mediawiki/2010/f/ff/IC_Team24.jpg"><img src="https://static.igem.org/mediawiki/2010/f/ff/IC_Team24.jpg" width="200"/></a>
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| - | </html>
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| - | |Maddie
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| | </div> | | </div> |
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| - | <a href="https://static.igem.org/mediawiki/2010/6/63/IC_Team26.jpg"><img src="https://static.igem.org/mediawiki/2010/6/63/IC_Team26.jpg" width="200"/></a>
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| - | </html>
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| - | |-
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| - | |Nick again
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| - | </div>
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| - | |}
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| | |<div ALIGN=CENTER> | | |<div ALIGN=CENTER> |
| | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5"; | | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5"; |
| Photos
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Logos
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| Our Imperial College iGEM Logo
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| PARASIGHT
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| Another really cool logo
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|
The Team
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| The team with advisors Chris and Tom
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| The Imperial College iGEM Team
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| Some team members with SynBio icon Jay Keasling!!!
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| Maddie and a bit of Kirill in labs
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| Maddie in labs again
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| Nick
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| Maddie in labs... AGAIN
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| Kirill, Maddie and Nick
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|
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| Nick (posing?)
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|
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| Piotr focusing, Anita not so much!
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| Piotr and Anita
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Brainstorming
|
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| Fv-fragment guided input module
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| Ideas on parasite detection
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| Nick's idea on cell population control
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| Nick's idea...
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| Nick's idea...
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| Nick's idea...
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Wet Lab
|
Surface Protein
|
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| Surface Protein Team Logo
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| Assembly Strategy for LytC
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| Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
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| Gel of PCR confirming pSB1C3 has been amplified.
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| Picture estimating the concentration of purified BOO14 and pSB1C3.
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| Colony PCR to detect sucessfully transformed bacteria.
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| 3 PCR reactions to determine the most effective protocol to amplify the CWB.
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| Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
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| Isolation of the promoter pVEG by gel purification.
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| Determining the ratio of pSB1C3 to pVEG for ligation.
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| Determining the ratio of lytC to pSB1C3 for ligation.
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|
Vectors
|
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| Vectors Team Logo
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| Assembly strategy for the AmyE vector
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| Assembly strategy for the PyrD vector
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| K14002 and oligo assembly
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| K143070 and K11064 assembly step
|
|
XylE
|
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| XylE Team Logo
|
|
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| Team XylE
|
|
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| The J23101 gene in a biobrick vector containg RFP gene.
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|
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| Midi-prep XylE digestion with xbaI and PstI
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| Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
|
|
|
| Catechol assay (before)
|
|
.jpg)
|
| Catechol assay (after) - Can you spot the difference?
|
|
|
| Catechol assay on XylE-trasformed cells in a 96-well plate
|
|
|
| First Successful Catechol Assay!
|
|
|
| First Successful Catechol Assay!
|
|
|
| :)
|
|
|
| Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
|
|
|
| The coloured pigment is transported out of the cells.
|
|
|
| Spectra of XylE transformed E.coli after addition of catechol assay
|
|
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| Catechol Assay cuvette setup
|
|
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| Catechol Assay cuvette setup
|
|
Strategy
|
|
| The full assembly strategy
|
|
|
| Wolf's primers
|
|
Parts
|
|
| BioBricks
|
|
|
| ComE binding sites in the promoters of the comCDE and comAB operons
|
|
Detection Module
|
|
| Dr Martha Betson explaining the Schistosoma lifecycle
|
| | |
Fast Response Module
|
|
| Results of a BLAST search using ComE (Accession no. AAC44897)
|
|
|
| Results of a BLAST search using BlpR (Accession no. CAC03515.1)
|
| |
| | |
| | |
| | |
| |
Output Module
|
| | |
Human Practices
|
|
| Human Practices panel: advisors
|
|
|
| The team looks reeeeaaaalllly interested
|
|
|
| Maddie's notes from the HP panel
|
|
|
| Notes...
|
|
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| Notes...
|
|
School Workshops
|
|
| Brainstorming
|
|
|
| Chameleon: Now you see me - now you don't
|
|
|
| More brainstorming
|
|
|
| ...and more brainstorming
|
|
|
| Ideas on regulation of Synthetic Biology
|
|
|
| H'n'H sauce (Hot&Healthy) - yummy
|
|
|
| More ideas on regulations of SynBio
|
|
|
| Brainstorming again
|
|
|
| iGEM <3
|
|
|
| Check out the lens creating bacteria!
|
|
|
| More ideas...
|
|
|
| Happy Bacteria?!
|
|
|
| Regulations...
|
|
"
