Team:Caltech/Protocols

From 2010.igem.org

(Difference between revisions)
Line 3: Line 3:
__NOTOC__
__NOTOC__
* [[Competent Cells]]
* [[Competent Cells]]
-
* [[Transformation|Transformation by Electroporation]]
+
* [[Electroporation|Transformation by Electroporation]]
* Making stock solutions
* Making stock solutions
* Freezer (glycerol) stocks
* Freezer (glycerol) stocks

Revision as of 23:29, 14 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png
  • Competent Cells
  • Transformation by Electroporation
  • Making stock solutions
  • Freezer (glycerol) stocks
  • Plating antibiotics
  • Sequencing primers
  • Making synthase casette
    • Sources of sequences
    • Optimizing & checking genes
  • Chemical/Crosslinking reactions
    • Digesting substrate (hydrolysis) + epoxidation (MCPBA)
    • crosslinking (AIBN3) + UV initiation
  • Inducing lysis via 632nm light initiation
  • Making cell/glycerol/agar mixture
    • Applying this to plates
  • Growing cells with PHA (and substrate oil)
  • CPCR program & protocol
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page

Retrieved from "http://2010.igem.org/Team:Caltech/Protocols"