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Team:UPO-Sevilla/Biobricks/Devices
From 2010.igem.org
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<h2>Device 1. Type: protein generator</h2> | <h2>Device 1. Type: protein generator</h2> | ||
<img class="device" src="https://static.igem.org/mediawiki/2010/6/66/BacterialCrowdingDevice01.png" alt="Bacterial Crowding Device 01"/> | <img class="device" src="https://static.igem.org/mediawiki/2010/6/66/BacterialCrowdingDevice01.png" alt="Bacterial Crowding Device 01"/> | ||
| - | <p><strong>Notes:</strong></p> | + | <p><strong>Notes:</strong> This device includes all Prh system proteins we synthesized using Mr. Gene services. Finally it was not built. </p> |
<h2>Device 2. Type: protein generator</h2> | <h2>Device 2. Type: protein generator</h2> | ||
<img class="device" src="https://static.igem.org/mediawiki/2010/1/16/BacterialCrowdingDevice02.png" alt="Bacterial Crowding Device 02"/> | <img class="device" src="https://static.igem.org/mediawiki/2010/1/16/BacterialCrowdingDevice02.png" alt="Bacterial Crowding Device 02"/> | ||
| - | <p><strong>Notes:</strong> We decided not to build this divice due to the fact that it already exists in <i> E. coli </i>K12 strain.</p> | + | <p><strong>Notes:</strong> This device includes all Fec system proteins we wanted to synthesize by PCR and SDM methods. We decided not to build this divice due to the fact that it already exists in <i> E. coli </i>K12 strain.</p> |
<h2>Device 3. Type: protein generator</h2> | <h2>Device 3. Type: protein generator</h2> | ||
<img class="device" src="https://static.igem.org/mediawiki/2010/0/04/BacterialCrowdingDevice03.png" alt="Bacterial Crowding Device 03"/> | <img class="device" src="https://static.igem.org/mediawiki/2010/0/04/BacterialCrowdingDevice03.png" alt="Bacterial Crowding Device 03"/> | ||
| - | <p><strong>Notes:</strong></p> | + | <p><strong>Notes:</strong> This device was created to perform an hybrid Fec/Prh system. Instead of it we built device 17 which also allows to use circuit 3.</p> |
<h2>Device 4. Type: protein generator</h2> | <h2>Device 4. Type: protein generator</h2> | ||
<img class="device" src="https://static.igem.org/mediawiki/2010/d/dd/BacterialCrowdingDevice04.png" alt="Bacterial Crowding Device 04"/> | <img class="device" src="https://static.igem.org/mediawiki/2010/d/dd/BacterialCrowdingDevice04.png" alt="Bacterial Crowding Device 04"/> | ||
| - | <p><strong>Notes:</strong></p> | + | <p><strong>Notes:</strong> We were not confident of circuit 4 function and we had not time to lose. That means that we did not assembly required parts for device 4.</p> |
<h2>Device 5. Type: reporter</h2> | <h2>Device 5. Type: reporter</h2> | ||
<img class="device" src="https://static.igem.org/mediawiki/2010/5/54/BacterialCrowdingDevice05.png" alt="Bacterial Crowding Device 05"/> | <img class="device" src="https://static.igem.org/mediawiki/2010/5/54/BacterialCrowdingDevice05.png" alt="Bacterial Crowding Device 05"/> | ||
| - | <p><strong>Notes:</strong></p> | + | <p><strong>Notes:</strong> We focused in circuit 3, which uses P<i>fecA</i> promoter, so we did not continue building any device with P<i>prhJ</i> promoter; although we synthesized P<i>prhJ</i> promoter using Mr. Gene services. </p> |
<h2>Device 6. Type: reporter</h2> | <h2>Device 6. Type: reporter</h2> | ||
Revision as of 11:37, 14 October 2010
Devices
In this section all devices we designed at the beginning of the summer are shown in a schematic way. You can see parts which make up each device and required inputs to perform each particular output. Another important information is the use of each device. Finally we present the optimized assembly strategy we followed to try to build these devices.
As you can see, we wanted to assembly a lot of parts, too much devices for our first time. This is why we had to focus in some devices and to forget assembling others. You will see a yellow star in the upper left corner of the prioritized devices. These are the devices we finally assembled.
Device 1. Type: protein generator
Notes: This device includes all Prh system proteins we synthesized using Mr. Gene services. Finally it was not built.
Device 2. Type: protein generator
Notes: This device includes all Fec system proteins we wanted to synthesize by PCR and SDM methods. We decided not to build this divice due to the fact that it already exists in E. coli K12 strain.
Device 3. Type: protein generator
Notes: This device was created to perform an hybrid Fec/Prh system. Instead of it we built device 17 which also allows to use circuit 3.
Device 4. Type: protein generator
Notes: We were not confident of circuit 4 function and we had not time to lose. That means that we did not assembly required parts for device 4.
Device 5. Type: reporter
Notes: We focused in circuit 3, which uses PfecA promoter, so we did not continue building any device with PprhJ promoter; although we synthesized PprhJ promoter using Mr. Gene services.
Device 6. Type: reporter
Notes: PRIORITIZED DEVICE!
Device 7. Type: Protein generator/Signal sender
Notes:
Device 8. Type: Protein generator/Signal sender
Notes: PRIORITIZED DEVICE!
Device 9. Type: Protein generator/Signal sender
Notes:
Device 10. Type: Protein generator/Signal sender
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 11. Type: Protein generator/Signal sender
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 12. Type: Protein generator/Signal sender
Notes: Finally we chose aspartate like the best attractant for E. coli. This is why Glutamate Synthase divices were not going to continue building.
Device 13. Type: Protein generator/Signal sender
Notes:
Device 14. Type: Protein generator/Signal sender
Notes: PRIORITIZED DEVICE!
Device 15. Type: Protein generator/Signal sender
Notes:
Device 16. Type: Constitutive protein generator/reporter
Notes:
Device 17. Type: Protein generator
Notes: PRIORITIZED DEVICE!
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