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Team:UPO-Sevilla/Notebook/08 06
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<p><strong>Paola Gallardo.</strong> Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p> | <p><strong>Paola Gallardo.</strong> Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p> | ||
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<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
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| + | <p>We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps are: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.</p> | ||
<p>Minpreps using inocula we put yesterday. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 & UPO11+2.</p> | <p>Minpreps using inocula we put yesterday. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 & UPO11+2.</p> | ||
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<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Revision as of 19:55, 23 October 2010
August, 6th
Production Team
Paola Gallardo. Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.
Assembly Team
We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps are: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.
Minpreps using inocula we put yesterday. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 & UPO11+2.
Return to Notebook
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