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Team:Newcastle/solution
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=== Parameters === | === Parameters === | ||
Revision as of 10:05, 17 June 2010
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Contents |
Required BioBricks
BioBrick 2 (End of crack & signalling system)
BioBrick 3 (Non-target-environment kill switch)
BioBrick 4 (Spider silk & Filamentous Cells)
BioBrick 5 (Glue & lysis)
Secretion Systems
Characteristics / Actions
- Alkaline resistant
- Motility
- Sense Oxygen (for cracks)
- Quorum Sensing
- UV Sensitivity
- Produce glue/Silk
- Produce product in response to signal
Products
- Spider silk
- Glue
- Amyloid Fiber (forms Beta-Sheet)
- Calcium Carbonate
- Quorum Sensing Peptides
- Surfactin
Priorities
- Filamentous cells
- CaCO3
- Quorum Sensing
- Spider silk
- Glue
- Directed movement
Types of Bacillus cells
- Motile rod cells
- Filamentous
- Chain of rods
- Spores
- Lysed
- Biofilm
- Dividing cell
Environment
- Fermenter (Batch)
- External Face of Concrete
- Inside Crack
- Spray
- End of crack
- Non-target
Experiments
- Movement into cracks
- Speed of swimming in a particular direction
Models
- Rate of migration down micro crack
- Rate of protein production
- Mechanical model of repair strength
- Whole process. Signal propagation to release glue
Parameters
- Speed of swimming in a particular direction (wt)
- Quantities of glue, s.s, CaCO3, cell density
- Strength (tensile) of bonding agents, filamentous cells
???
- Grown in fermenter, rich medium
- Rods
- Sporulation medium
- spores
- Spray canister
- spores
- Sprayed on concrete surface
- germinate -> rods
- In crack
- rods
- End of crack
- Produce glue/CaCO3/silk/filaments
- Produce signal peptides
