Team:Newcastle/Filamentous Cells
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
=Filamentous Cells= | =Filamentous Cells= | ||
+ | ==Filamentous cells genes list== | ||
+ | {| | ||
+ | |- | ||
+ | |''yneA'' (Transcribed with ''yneB'', ''ynzC'', is an analogue of ''sulA'' in ''E.coli'') * our biobrick is designed to over express this gene r educing cell division possibly by inhibiting FtsZ ring formation or constriction. | ||
+ | |- | ||
+ | |''dinR'' (Homologue of ''lexA'' in ''E.coli'' transcribed in the opposite direction) | ||
+ | |- | ||
+ | |''ftsZ'' (Involved in the recruitment of other proteins to the divisisome for cytokinesis, strangely over expression results in disruption of Zring formation as well as reduced expression) | ||
+ | |- | ||
+ | |''secA'' (Involved in the secretion of extracellular proteins and the insertion of transmembrane proteins) | ||
+ | |- | ||
+ | | ''recA'' (Involved in SOS response removing the repressor DinR (LexA)) | ||
+ | |- | ||
+ | |''wpr'' and ''epr'' produce extracellular proteases that cleave the signal peptide/transmembrane domain of YneA | ||
+ | |- | ||
+ | |''ezr'' produces a protein which sequesters FtsZ monomer by binding its C terminal domain and also inhibits GTP binding; however overexpression does not result in filamentation. | ||
+ | |- | ||
+ | | ''min C,D ,J and divIVA'' prevent polar cell division . | ||
+ | |- | ||
+ | |Positive regulators of FtsZ: ''ftsA, zapA, zipA, ftsL and divIC'' | ||
+ | |- | ||
+ | |Inhibitors of Daughter cell separation: ''lytC,D,E,F and cwlS'' *Chains rather than filaments, ''yneA'' is also reported to increase the time spent in chains well into the stationary phase of bacterial growth. | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
===YneA=== | ===YneA=== | ||
{| | {| | ||
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| [[Image:Coding region.jpg]] | | [[Image:Coding region.jpg]] | ||
|- | |- | ||
- | |YneA suppressed in wt without SOS induction | + | |YneA suppressed in wt without SOS induction # |
|- | |- | ||
|Expression of YneA from IPTG controlled promoter in wt leads to elongation. | |Expression of YneA from IPTG controlled promoter in wt leads to elongation. |
Revision as of 12:57, 19 June 2010
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Contents |
Filamentous Cells
Filamentous cells genes list
yneA (Transcribed with yneB, ynzC, is an analogue of sulA in E.coli) * our biobrick is designed to over express this gene r educing cell division possibly by inhibiting FtsZ ring formation or constriction. |
dinR (Homologue of lexA in E.coli transcribed in the opposite direction) |
ftsZ (Involved in the recruitment of other proteins to the divisisome for cytokinesis, strangely over expression results in disruption of Zring formation as well as reduced expression) |
secA (Involved in the secretion of extracellular proteins and the insertion of transmembrane proteins) |
recA (Involved in SOS response removing the repressor DinR (LexA)) |
wpr and epr produce extracellular proteases that cleave the signal peptide/transmembrane domain of YneA |
ezr produces a protein which sequesters FtsZ monomer by binding its C terminal domain and also inhibits GTP binding; however overexpression does not result in filamentation. |
min C,D ,J and divIVA prevent polar cell division . |
Positive regulators of FtsZ: ftsA, zapA, zipA, ftsL and divIC |
Inhibitors of Daughter cell separation: lytC,D,E,F and cwlS *Chains rather than filaments, yneA is also reported to increase the time spent in chains well into the stationary phase of bacterial growth. |
YneA
Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.