Team:Harvard/flavor/parts

From 2010.igem.org

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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
-
<tr><td>J45004_F</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
+
<tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td><td></td></tr>
-
<tr><td>J45004_R</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
+
<tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td><td></td></tr>
-
<tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
+
<tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td><td></td></tr>
-
<tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
+
<tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td><td></td></tr>
<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td></td></tr>
<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td></td></tr>
<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td></td></tr>
<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td></td></tr>
-
<tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
+
<tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td><td></td></tr>
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr>
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr>
<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td></td></tr>
<tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td></td></tr>

Revision as of 23:39, 11 October 2010


parts and primers

Primers

NameDescriptionSequence
J45004_FWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer
J45004_RWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer
J45017_FWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer
J45017_RWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer
NOSterm_BB_FPlant specific terminator forward primer
NOSterm_BB_RPlant specific terminator reverse primer
Stop_NOSterm_BB_FNOSt plant specific terminator with stop codon on end
pENTCUP2_BB_FPlant specific promoter forward primer
pENTCUP2_BB_RPlant specific promoter reverse primer