"
Team:Harvard/flavor/parts
From 2010.igem.org
(Difference between revisions)
| Line 36: | Line 36: | ||
<tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr> | ||
<tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
| - | <tr><td>NOSterm_BB_F</td><td> | + | <tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td></td></tr> |
| - | <tr><td>NOSterm_BB_R</td><td> | + | <tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td></td></tr> |
<tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | <tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | ||
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr> | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr> | ||
Revision as of 15:41, 10 October 2010
parts and primers
Primers
| Name | Description | Sequence |
| J45004_F | HindIII-pENTCUP2 forward primer for Expression Series insert | |
| J45004_R | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
| J45017_F | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
| J45017_R | gusA-NheI reverse primer for Reporter Series 1 insert | |
| NOSterm_BB_F | Plant specific terminator forward primer | |
| NOSterm_BB_R | Plant specific terminator reverse primer | |
| Stop_NOSterm_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| pENTCUP2_BB_F | Plant specific promoter forward primer | |
| pENTCUP2_BB_R | Plant specific promoter reverse primer |
