Template:Leanna newesttoggle
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<table width=70%><tr><td><div id="bodybaby">the bacterial uv toggle</div></td> | <table width=70%><tr><td><div id="bodybaby">the bacterial uv toggle</div></td> | ||
<tr><td><br>In the beginning, there was a UV Toggle | <tr><td><br>In the beginning, there was a UV Toggle | ||
- | (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good,<a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style="float: left; display: inline;" height= | + | (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. <a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style="float: left; display: inline;" height=100px src="https://static.igem.org/mediawiki/2010/8/80/First.png"></a>The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by UV exposure -- the first image.<br> |
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Revision as of 03:29, 10 October 2010
Undergraduates Instructors Advisors Fun Sponsors
Overview Toggle Phage Mammalian Summary Acknowledgements
Materials & Methods Biosafety Journal Club
the bacterial uv toggle |
In the beginning, there was a UV Toggle (Collins, 2000). The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. ![]() Then the team decided to make the signal amplify itself, and record a movie to see its propogation. And it was so. But the team noticed a circle of cell death where the UV exposure had killed some of the cells in the lawn. They decided to make a pLPTa, a low power toggle that still provided bistability, but required less UV power to induce a toggle switch. By site-directed mutagenesis, the team changed the lambda repressor (cI) gene in the Collins pTSMa to a cI that is more sensitive to cleavage by Rec-A, the enzyme activated by UV light exposure. |