Template:Leanna newesttoggle
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<table width=70%><tr><td><div id="bodybaby">the bacterial uv toggle</div></td> | <table width=70%><tr><td><div id="bodybaby">the bacterial uv toggle</div></td> | ||
<tr><td><br>In the beginning, there was a UV Toggle | <tr><td><br>In the beginning, there was a UV Toggle | ||
- | (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good, | + | (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good,<a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style="float: left; display: inline;" height=200px src="https://static.igem.org/mediawiki/2010/8/80/First.png"></a> and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by UV exposure -- the first image.<br> |
- | <a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style=" | + | <br> <br> |
- | Then the team decided to make the signal amplify itself, <object width="300" height="180" style="display: inline; margin: 0px 16%;"><param name="movie" value="http://www.youtube.com/v/6KHabnSzCJ8?fs=1&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/6KHabnSzCJ8?fs=1&hl=en_US" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="400" height="240"></embed></object | + | Then the team decided to make the signal amplify itself, and record a movie to see its propogation. And it was so.<br><object width="300" height="180" style="display: inline; margin: 0px 16%;"><param name="movie" value="http://www.youtube.com/v/6KHabnSzCJ8?fs=1&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/6KHabnSzCJ8?fs=1&hl=en_US" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="400" height="240"></embed></object> |
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Revision as of 03:29, 10 October 2010
Undergraduates Instructors Advisors Fun Sponsors
Overview Toggle Phage Mammalian Summary Acknowledgements
Materials & Methods Biosafety Journal Club
the bacterial uv toggle |
In the beginning, there was a UV Toggle (Collins, 2000). The 2010 MIT iGEM team saw that it was good, ![]() Then the team decided to make the signal amplify itself, and record a movie to see its propogation. And it was so. But the team noticed a circle of cell death where the UV exposure had killed some of the cells in the lawn. They decided to make a pLPTa, a low power toggle that still provided bistability, but required less UV power to induce a toggle switch. By site-directed mutagenesis, the team changed the lambda repressor (cI) gene in the Collins pTSMa to a cI that is more sensitive to cleavage by Rec-A, the enzyme activated by UV light exposure. |