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Team:Edinburgh/Notebook/Blue light producer
From 2010.igem.org
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'''29/7/2010''' | '''29/7/2010''' | ||
| + | |||
| + | '''12/8/2010''' | ||
| + | update on LumP: | ||
| + | * still getting pink/red colonies from transformants | ||
| + | * single digests give a band of about 3kb | ||
| + | * double digests give a band of about 2.5 kb -highly confusing | ||
| + | * and also, no sign on RFP despite red/pink growth. | ||
| + | * Vector wa ssequenced and LumP is definately there. | ||
| + | |||
| + | '''gel pohot no x and y''' + description. | ||
Revision as of 12:28, 7 October 2010
Blue Light Producer
13/7/2010
Empty edinbrick vector for control.
- LuxAB + edinbrick ligation.
28/7/2010
an update on LuxAB:
- The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
- Still have some digested LuxAB-ediI so re-did ligation and transformation.
Gel Pic- minipreps
| Lane 1&15 | Lane 2 | Others |
| Ladder | EdiI | LuxAB |
GEL PIC
| Lane 1 | Lane 2 +3 | Lanes 3-6 |
| Ladder | EdiI | LuxAB |
- The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working
29/7/2010
12/8/2010 update on LumP:
- still getting pink/red colonies from transformants
- single digests give a band of about 3kb
- double digests give a band of about 2.5 kb -highly confusing
- and also, no sign on RFP despite red/pink growth.
- Vector wa ssequenced and LumP is definately there.
gel pohot no x and y + description.
