Team:Edinburgh/Notebook/Blue light producer

From 2010.igem.org

(Difference between revisions)
(→Blue Light Producer)
(→Blue Light Producer)
Line 118: Line 118:
Empty edinbrick vector for control.  
Empty edinbrick vector for control.  
* LuxAB + edinbrick ligation.
* LuxAB + edinbrick ligation.
 +
 +
'''28/7/2010'''
 +
 +
an update on LuxAB:
 +
*The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
 +
*Still have some digested LuxAB-ediI so re-did ligation and transformation.
 +
'''Gel Pic- minipreps'''
 +
 +
{| border="1"
 +
| Lane 1&15
 +
| Lane 2
 +
| Others
 +
|-
 +
| Ladder
 +
| EdiI
 +
| LuxAB
 +
|}

Revision as of 11:57, 7 October 2010





Blue Light Producer


13/7/2010

Empty edinbrick vector for control.

  • LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

  • The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
  • Still have some digested LuxAB-ediI so re-did ligation and transformation.

Gel Pic- minipreps

Lane 1&15 Lane 2 Others
Ladder EdiI LuxAB
Retrieved from "http://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_producer"