Team:Cambridge/Gibson/Protocol

Step 1) Design Primers

• If you wish to ligate two pieces of DNA using Gibson they must be altered so as to have 40bp of overlap with respect to each other.

//Insert diagram of two pieces of DNA with overlap

• The standard way to do this is with PCR with specialised primers

• We have designed a tool to help you do this: [http://www.gibthon.org Gibthon]

Step 2) Order Primers

• This step can take a while, so Gibson Assembly requires some planning ahead

Step 3) PCR

Step 4) Gibson Assembly

Master mix