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'''will include intro image, idea is currently being fleshed out from sketch into image''' | '''will include intro image, idea is currently being fleshed out from sketch into image''' | ||
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| - | * | + | =21st century antibiotics GENERAL STORYLINE FLOW= |
| - | *computational | + | '''The bullet points are the key images that need to be generated. They should be very general and easy to understand! The text will basically be explaning the pictures and telling the story.... think of this as a comic book! |
| - | + | ''' | |
| - | *mutants | + | |
| - | * | + | ==gram(+)== |
| - | + | ===summary=== | |
| + | *why anthrax is bad | ||
| + | *proteins fighting anthrax | ||
| + | ===design=== | ||
| + | *circular permutation | ||
| + | *computational design | ||
| + | ===build=== | ||
| + | *How we made mutants | ||
| + | *How we made protiein | ||
| + | ===Test=== | ||
*gel/mass spec cp vs D | *gel/mass spec cp vs D | ||
*activity cp vs D | *activity cp vs D | ||
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*CP mutations | *CP mutations | ||
*Specificity switch | *Specificity switch | ||
| + | ===Future Directions=== | ||
| + | *Just some bullet points, no figures needed | ||
| - | + | ==gram(-)== | |
| + | ===summary (bugs vs bugs)=== | ||
| + | *type 6 secretion (poison spear) | ||
| + | *target (make toxin that induces in prescence of XXX) | ||
| + | ===design=== | ||
| + | *operon in Pseudomonas —> operon in e coli | ||
| + | **what parts of this operon are needed? | ||
| + | *sensor —produces—> toxin/antitoxin (classic tinkercell picture) | ||
| + | ===build=== | ||
| + | *exporter —> recombineering to promoter | ||
| + | *sensor/toxin: —> PCR —> HSL biobrick vector | ||
| + | ===Test=== | ||
| + | *Exporter: Still under construction | ||
| + | *Sensor/Toxin | ||
| + | **Toxin is toxic in ecoli; Antidote still works in e.coli; Sensor works and produces system as expected! | ||
| + | ===Future Directions=== | ||
| + | *Just some quick Bullet Points | ||
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Revision as of 02:35, 30 September 2010
Note: this is a very crude sketch that will be (likely) turned into a final banner
Antibiotics for the 21st Century
While vital to our quality of life, traditional antibiotics face the serious problems of widespread bacterial resistance and destruction of natural gut flora - problems which call for improved twenty-first century antibiotics. Using synthetic biology tools, we designed, built, and tested two new systems to fight infections by both broad types of bacteria - Gram-positive and Gram-negative. Our first project targets Bacillus anthracis, the Gram-positive pathogen that causes anthrax. We re-engineered an enzyme to remove the pathogen's protective coating, rendering it defenseless against the immune system. In our second project, we re-engineered and transplanted a protein secretion system capable of combating Gram-negative bacteria into E. coli. This system was designed to target Gram-negative pathogens in a modular and controllable fashion. These two systems are the vanguard of a new era of antibiotics using the power of nature harnessed with the tools of synthetic biology.
will include intro image, idea is currently being fleshed out from sketch into image
21st century antibiotics GENERAL STORYLINE FLOW
The bullet points are the key images that need to be generated. They should be very general and easy to understand! The text will basically be explaning the pictures and telling the story.... think of this as a comic book!
gram(+)
summary
- why anthrax is bad
- proteins fighting anthrax
design
- circular permutation
- computational design
build
- How we made mutants
- How we made protiein
Test
- gel/mass spec cp vs D
- activity cp vs D
- catalytic KO of CP vs CP
- CP mutations
- Specificity switch
Future Directions
- Just some bullet points, no figures needed
gram(-)
summary (bugs vs bugs)
- type 6 secretion (poison spear)
- target (make toxin that induces in prescence of XXX)
design
- operon in Pseudomonas —> operon in e coli
- what parts of this operon are needed?
- sensor —produces—> toxin/antitoxin (classic tinkercell picture)
build
- exporter —> recombineering to promoter
- sensor/toxin: —> PCR —> HSL biobrick vector
Test
- Exporter: Still under construction
- Sensor/Toxin
- Toxin is toxic in ecoli; Antidote still works in e.coli; Sensor works and produces system as expected!
Future Directions
- Just some quick Bullet Points
