Team:UCL London/Our BioBricks

From 2010.igem.org

(Difference between revisions)
Line 90: Line 90:
| pSB1A2
| pSB1A2
| 3385
| 3385
 +
|-
 +
| B0015
 +
| Double Terminator
 +
| Plate1_23L
 +
| pSB1AK3
 +
| 129
|-
|-
-
|  
+
| pSB1K3
-
| row 6, cell 2
+
| Kanamycin Resistance
-
| row 6, cell 3
+
| Plate1_5A
-
| row 6, cell 4
+
| pSB1K3+J04450 insert
-
| row 6, cell 5
+
| 2206
|-
|-
-
| row 7, cell 1
+
| pSB1T3
-
| row 7, cell 2
+
| Tetracycline Resistance
-
| row 7, cell 3
+
| Plate 1_7A
-
| row 7, cell 4
+
| pSB1T3+J04450 insert
-
| row 7, cell 5
+
| 2463
|-
|-
-
| row 8, cell 1
+
| pSB1C3
-
| row 8, cell 2
+
| Chloramphenicol Resistance
-
| row 8, cell 3
+
| Plate 1_3A
-
| row 8, cell 4
+
| pSB1C3+J04450 insert
-
| row 8, cell 5
+
| 2072
|-
|-
-
| row 9, cell 1
+
| R0011
-
| row 9, cell 2
+
| Promoter (lacI regulated, lambda pL hybrid)
-
| row 9, cell 3
+
| Plate1_6G
-
| row 9, cell 4
+
| pSB1A2
-
| row 9, cell 5
+
| 55
|-
|-
-
| row 10, cell 1
+
| B0034
-
| row 10, cell 2
+
| RBS
-
| row 10, cell 3
+
| Plate1_2M
-
| row 10, cell 4
+
| pSB1A2
-
| row 10, cell 5
+
| 12
|-
|-
-
| row 11, cell 1
+
| C0079
-
| row 11, cell 2
+
| lasR activator from P. aeruginosa PAO1(+LVA)
-
| row 11, cell 3
+
| Plate1_14J
-
| row 11, cell 4
+
| pSB2K3
-
| row 11, cell 5
+
| 756
|-
|-
-
| row 12, cell 1
+
| pSB1AK3
-
| row 12, cell 2
+
| Amp+Kana
-
| row 12, cell 3
+
| Plate1_11A
-
| row 12, cell 4
+
| +J04450 insert
-
| row 12, cell 5
+
| 3189
|-
|-
-
| row 13, cell 1
+
| pSB1AT3
-
| row 13, cell 2
+
| Amp+Tetra
-
| row 13, cell 3
+
| Plate1_13A
-
| row 13, cell 4
+
| +J04450 insert
-
| row 13, cell 5
+
| 3446
|-
|-
-
| row 14, cell 1
+
| pSB1AC3
-
| row 14, cell 2
+
| Amp+Chlora
-
| row 14, cell 3
+
| Plate1_9A
-
| row 14, cell 4
+
| +J04450 insert
-
| row 14, cell 5
+
| 3055
 +
|-
 +
| K274200
 +
| CrtEBIY with rbs (Orange)
 +
| Plate3_6L
 +
| pSB1A2
 +
| 4555
 +
|-
 +
| K274002
 +
| Purple
 +
| Plate3_12B
 +
| pSB1T3
 +
| 7345
 +
|-
 +
| K274003
 +
| Dark Green
 +
| Plate3_20H
 +
| pSB1K3
 +
| 6032
|-
|-
| row 15, cell 1
| row 15, cell 1
| row 15, cell 2
| row 15, cell 2
-
| row 15, cell 3
+
| Plate3_20D
-
| row 15, cell 4
+
| pSB1T3
-
| row 15, cell 5
+
| 6032
 +
|-
 +
| K274004
 +
| Light Green
 +
| Plate3_20J
 +
| pSB1K3
 +
| 6200
 +
|-
 +
|
 +
|
 +
| Plate3_22F
 +
| pSBT3
 +
| 6200
 +
|-
 +
| I712069
 +
| RBS+T7 RNAP
 +
| Plate2_13K
 +
| pSB1AK3
 +
|
 +
|-
 +
| I719005
 +
| T7 promoter
 +
| Plate1_15N
 +
| pSB1A2
 +
|
 +
|-
 +
| I712074
 +
| T7 strong pro
 +
| Plate1_6N
 +
| pSB1AK8
 +
|
 +
|-
 +
| I13507
 +
| RBS+RFP+T
 +
| Pate1_22O
 +
| pSB1A2
 +
| 861
|-
|-
-
 
|}
|}

Revision as of 11:29, 29 September 2010

UCL IGEM 2010

RETURN TO IGEM 2010

Parts Used

Part Code Part Name Location Backbone Length(bp)
R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
K239005 Nark Eurogentec Amp 139
K239006 mNark Eurogentec Amp 89
R0079 Promoter (LasR & PAI regulated) Plate1_19P pSB1A2 936
K274100 VRDP Plate3_6H pSB1A2 3385
B0015 Double Terminator Plate1_23L pSB1AK3 129
pSB1K3 Kanamycin Resistance Plate1_5A pSB1K3+J04450 insert 2206
pSB1T3 Tetracycline Resistance Plate 1_7A pSB1T3+J04450 insert 2463
pSB1C3 Chloramphenicol Resistance Plate 1_3A pSB1C3+J04450 insert 2072
R0011 Promoter (lacI regulated, lambda pL hybrid) Plate1_6G pSB1A2 55
B0034 RBS Plate1_2M pSB1A2 12
C0079 lasR activator from P. aeruginosa PAO1(+LVA) Plate1_14J pSB2K3 756
pSB1AK3 Amp+Kana Plate1_11A +J04450 insert 3189
pSB1AT3 Amp+Tetra Plate1_13A +J04450 insert 3446
pSB1AC3 Amp+Chlora Plate1_9A +J04450 insert 3055
K274200 CrtEBIY with rbs (Orange) Plate3_6L pSB1A2 4555
K274002 Purple Plate3_12B pSB1T3 7345
K274003 Dark Green Plate3_20H pSB1K3 6032
row 15, cell 1 row 15, cell 2 Plate3_20D pSB1T3 6032
K274004 Light Green Plate3_20J pSB1K3 6200
Plate3_22F pSBT3 6200
I712069 RBS+T7 RNAP Plate2_13K pSB1AK3
I719005 T7 promoter Plate1_15N pSB1A2
I712074 T7 strong pro Plate1_6N pSB1AK8
I13507 RBS+RFP+T Pate1_22O pSB1A2 861


Parts Submitted

Part Code Part Name Location Backbone Length(bp)
R0010 PROMOTER (lacI Regulated) Plate1_1D pSB1A2 200
K239005 Nark Eurogentec Amp 139
row 3, cell 1 row 3, cell 2 row 3, cell 3 row 3, cell 4 row 3, cell 5
row 4, cell 1 row 4, cell 2 row 4, cell 3 row 4, cell 4 row 4, cell 5
row 5, cell 1 row 5, cell 2 row 5, cell 3 row 5, cell 4 row 5, cell 5
row 6, cell 1 row 6, cell 2 row 6, cell 3 row 6, cell 4 row 6, cell 5
row 7, cell 1 row 7, cell 2 row 7, cell 3 row 7, cell 4 row 7, cell 5
row 8, cell 1 row 8, cell 2 row 8, cell 3 row 8, cell 4 row 8, cell 5
row 9, cell 1 row 9, cell 2 row 9, cell 3 row 9, cell 4 row 9, cell 5
row 10, cell 1 row 10, cell 2 row 10, cell 3 row 10, cell 4 row 10, cell 5
row 11, cell 1 row 11, cell 2 row 11, cell 3 row 11, cell 4 row 11, cell 5
row 12, cell 1 row 12, cell 2 row 12, cell 3 row 12, cell 4 row 12, cell 5
row 13, cell 1 row 13, cell 2 row 13, cell 3 row 13, cell 4 row 13, cell 5
row 14, cell 1 row 14, cell 2 row 14, cell 3 row 14, cell 4 row 14, cell 5
row 15, cell 1 row 15, cell 2 row 15, cell 3 row 15, cell 4 row 15, cell 5


Retrieved from "http://2010.igem.org/Team:UCL_London/Our_BioBricks"