Team:Edinburgh/BioBricks

From 2010.igem.org

(Difference between revisions)
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   <td><b>cat</b></td>
   <td><b>cat</b></td>
   <td>Protein Domain</td>
   <td>Protein Domain</td>
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   <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K322210">BBa_K322210</a></td>
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   <td><a href="http://partsregistry.org/Part:BBa_K322210">BBa_K322210</a></td>
   <td>chloramphenicol acetyltransferase (chloramphenicol resistance gene)</td>
   <td>chloramphenicol acetyltransferase (chloramphenicol resistance gene)</td>
  </tr>
  </tr>
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   <td><b>sacB</b></td>
   <td><b>sacB</b></td>
   <td>Coding</td>
   <td>Coding</td>
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   <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K322921">BBa_K322921</a></td>
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   <td><a href="http://partsregistry.org/Part:BBa_K322921">BBa_K322921</a></td>
   <td>Bacillus subtilis levansucrase, lethal to <i>E. coli</i> in presence of sucrose</td>
   <td>Bacillus subtilis levansucrase, lethal to <i>E. coli</i> in presence of sucrose</td>
  </tr>
  </tr>
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<br>
<br>
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<p><b>Biobrick #1</b></p>
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<p>***</p>
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<p>A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.</p>
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<br>
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<p><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></p>
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<table border="0" cellspacing="10" cellpadding="5" style="background:transparent; border-color:transparent;">
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<tr>
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<p>Currently, this part is only present in sequence form. Physical DNA will be submitted shortly.</p>
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  <th width="100px"><u>Name</u></th>
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  <th width="100px"><u>Type</u></th>
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<p>Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. <i>Analytical Biochemistry</i> <b>366</b>: 131-136 </p>
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  <th width="100px"><u>Link</u></th>
 +
  <th><u>Description</u></th>
 +
<tr>
 +
  <td align="left"><b>Codon optimised bright firefly luciferase</b></td>
 +
  <td>Coding</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></td>
 +
  <td>Photinus pyralis luciferase mutated for increased bioluminescence.</td>
 +
</tr>
 +
</table>
<br>
<br>

Revision as of 13:58, 28 September 2010







BioBricks: Genomic BRIDGEs


For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).

Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!


Name Type Link Description
cat Protein Domain BBa_K322210 chloramphenicol acetyltransferase (chloramphenicol resistance gene)
sacB Coding BBa_K322921 Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose




BioBricks: Bacterial BRIDGEs


***


Name Type Link Description
Codon optimised bright firefly luciferase Coding BBa_K322451 Photinus pyralis luciferase mutated for increased bioluminescence.



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