Team:UC Davis/notebook/week8.html

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<li>Light sensor: light sensor may have been completed, as Parts A&B&C may have been ligated together.  However, we found that the complete part may be questionable, as when we digested them, we find that they are not the right lengths.  </li><br />
<li>Light sensor: light sensor may have been completed, as Parts A&B&C may have been ligated together.  However, we found that the complete part may be questionable, as when we digested them, we find that they are not the right lengths.  </li><br />
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<li>ON switch progress: All colonies turned red. .  Spooky transcription returs.  Will continue w/ part regardless.  Also, begin next portion: </li><br />
+
<li>ON switch progress: All colonies turned red. .  Spooky transcription returs.  Will continue w/ part regardless.  Also, begin next portion: </li>
-
 
+
<li>I12006-B0034-C0051-B0034-E1010-F1610</li>
<li>I12006-B0034-C0051-B0034-E1010-F1610</li>
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<li>To help explain part construction, the composite part of B0034-C0051-B0034-E1010-F1610 will be used: K327001</li>
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<li>I12006 will be cut with Spe & Pst; B0034-C0051-B0034-E1010-F1610 (also known as K327001) will be cut with Xba & pst</li><br />
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<li>I12006 will be cut with Spe & Pst; K327001 will be cut with Xba & pst</li><br />
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<li>OFF switch</li>
<li>OFF switch</li>

Latest revision as of 05:38, 27 September 2010

Week 8: 8/1/10 through 8/7/10

  • Light sensor: light sensor may have been completed, as Parts A&B&C may have been ligated together. However, we found that the complete part may be questionable, as when we digested them, we find that they are not the right lengths.

  • ON switch progress: All colonies turned red. . Spooky transcription returs. Will continue w/ part regardless. Also, begin next portion:
  • I12006-B0034-C0051-B0034-E1010-F1610
  • I12006 will be cut with Spe & Pst; B0034-C0051-B0034-E1010-F1610 (also known as K327001) will be cut with Xba & pst

  • OFF switch
  • Starting off with new digestions, attempting with the fast digest procedure
  • I12006 (ES)-K327001 (EX)
  • B0034 (ES)-C0052 (EX)-I746352 (SP)-B0034 (XP)-C0040 (ES)-B0015 (EX)

  • Initiator progress: Ligation of RBS-C0051-B0015 worked!

  • Week 8
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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