Team:UNIPV-Pavia/Calendar/September/settimana4

(Difference between revisions)

Revision as of 13:19, 24 September 2010

SEPTEMBER: WEEK 4

Screening PCR on 6 colonies picked from I55 was performed. The results were the follow:

I55.

Inoculum of I52, I53, I55, I56 and I57 in LB+Amp for MiniPrep of the following day

Miniprep and Nanodrop quantification for:

For I61 the DNA is already available quantified 147,1 ng/ul.

After 3 hour digestion was performed:

Tecan Test was performed on prepared samples, after the usual protocol (dilution, medium change and dilution 1:1000).

@@ Line 57: / Line 57: @@
 After 3 hour digestion was performed:
+{| border="1" align='center'
+|  ''Culture''    ||   ''Kind''   ||  ''Final reaction volume (ul) '' ||  ''DNA (ul)''  ||  ''H20 (ul)''  ||   ''Enzyme 1 (ul)''  ||  ''Enzyme 2 (ul)'' ||   ''Buffer H (ul)''
+|-
+|    I52  ||  Screening || 25 ||  10,8 ||  9,7   ||  1 EcoRI  || 1 XbaI   || 2,5
+|-
+|    I53  ||  Screening || 25 ||  8,5 ||  12   ||  1 EcoRI  || 1 XbaI   || 2,5
+|-
+|    I55  ||  Screening || 25 ||  14 ||  6,5   ||  1 EcoRI  || 1 SpeI   || 2,5
+|-
+|    I56  ||  Screening || 25 ||  10 ||  10,5   ||  1 XbaI  || 1 PstI   || 2,5
+|-
+|    I57  ||  Screening || 25 ||  9,7 ||  10,8   ||  1 EcoRI  || 1 XbaI   || 2,5
+|-
+|    I61  ||  Screening || 25 ||  6,8 ||  13,7   ||  1 EcoRI  || 1 XbaI   || 2,5
+|}