Team:Newcastle/1 September 2010

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=Gel extraction of amplified plasmid pSB1C3 for ''rocF''=
 
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==Aim==
 
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The aim of this experiment is to perform gel extraction of the band containing the amplified fragment of the plasmid vector pSB1C3.
 
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==Materials and Protocol==
 
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Please refer to: [[Team:Newcastle/Gel extraction| Gel extraction]].
 
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==Result==
 
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For plasmid vector pSB1C3, we used 1.5% agarose gel and run the gel on 80 Volts for better resolution.
 
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We used 1Kb DNA ladder (Promega) for the gel containing plasmid vector.
 
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{|border=1
 
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!
 
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!'''Plasmid Vector pSB1C3'''
 
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|'''Size of the Fragment (in bp)'''
 
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|2072 approx.
 
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'''Table 1''': Table represents the size of the plasmid vector pSB1C3 represented as bands on the gel.
 
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#Weight of gel 0.3g, nanodrop value 27.3ng/microL
 
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#Weight of gel 0.4g, nanodrop value 21.6ng/microL
 
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==Discussion==
 
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We found a band of the appropriate size and cut it out and placed the part of the gel in a 2ml eppendorf tube.
 
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==Conclusion==
 
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Gel electrophoresis was successful and we did gel extraction of the fragment containing plasmid pSB1C3.
 
=Natto Rehydration=
=Natto Rehydration=
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==Aims==  
==Aims==  
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We aimed to rehydrate and plate the natto strain (''Bacillus subtilis ATCC 31578'') bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells.
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We aimed to rehydrate and plate the natto strain (''Bacillus subtilis ATCC 31578'') bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells. The cells are going to be used to test the strength of PGA glue.
==Materials==
==Materials==

Revision as of 22:44, 25 October 2010

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Natto Rehydration

Aims

We aimed to rehydrate and plate the natto strain (Bacillus subtilis ATCC 31578) bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells. The cells are going to be used to test the strength of PGA glue.

Materials

  • Pennassay G- Thy medium:
  1. Pennassay broth- 17.5g
  2. Glucose- 20g
  3. Thymine- 0.05g
  4. Distilled water- 1000ml
  • Pennassay broth( per l of water):
  1. peptone- 10g
  2. Yeast extract- 1.5g
  3. Sodium chloride- 3.5g
  4. Glucose- 1.0g
  5. Potassium phosphate dibasic- 3.68g
  6. Potassium phospahte monobasic- 1.32g
  7. Beef extract- 1.5g

ph7 store at 2-8 °C


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