Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/16
From 2010.igem.org
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'''Procedure''' | '''Procedure''' | ||
- | #Pour the Agarose gel | + | #Pour the Agarose gel onto gel plate and make wells on it. |
- | #Apply the 10×Loading buffer in the one of wells | + | #Apply the 10×Loading buffer in the one of wells. |
#Apply the Insert DNA in another well. | #Apply the Insert DNA in another well. | ||
Revision as of 06:25, 21 September 2010
2010/08/16 Monday(NEX)
Experiment:Make Agarose Gels Electro-phoresis
Member
NEX and watachin
Materials
- 1×TaE 200ml
- Agarose 2g
- Ethidium bromide 10µl
Procedure
- Add and mixture above materials in an Erlenmeyer flask
- Warm up them in a Microwave oven
Experiment:Agarose Gel Electro-phoresis
Member
Same avobe
Materials
- Insert DNA
- Agarose Gel
- 10×Loading buffer 5μl
Procedure
- Pour the Agarose gel onto gel plate and make wells on it.
- Apply the 10×Loading buffer in the one of wells.
- Apply the Insert DNA in another well.
Consequence
We could see the bcsB.