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Team:Edinburgh/BioBricks
From 2010.igem.org
(Difference between revisions)
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<ul> | <ul> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Project/Protocol">the protocol</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Protocol">the protocol</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh | + | <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">future work</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">future work</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_producer">green light</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_producer">green light</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_sensor">green sensor</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_sensor">green sensor</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial | + | <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">future work</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">future work</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li> | ||
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<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">future work</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">future work</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li> | ||
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<ul> | <ul> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">results</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li> |
<li><a href="https://2010.igem.org/Team:Edinburgh/Human">future work</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">future work</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Human">references</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">references</a></li> | ||
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<br> | <br> | ||
| - | <p>For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing <i>cat</i> (chloramphenicol resistance) and <i>sacB</i> (prevents growth on sucrose). | + | <p>For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing <i>cat</i> (chloramphenicol resistance) and <i>sacB</i> (prevents growth on sucrose).</p> |
| - | + | ||
| - | + | ||
| - | In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed <i>E. coli</i> no longer smells!</p> | + | <p>Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on <i>sacB</i>.</p> |
| + | |||
| + | <p>In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed <i>E. coli</i> no longer smells!</p> | ||
<br> | <br> | ||
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<br> | <br> | ||
| - | <p><b>Biobrick #1</b> | + | <p><b>Biobrick #1</b></p> |
| - | < | + | <p>A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.</p> |
| - | A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.< | + | |
| - | <a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a> | + | <p><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></p> |
| - | < | + | |
| - | Currently, this part is only present in sequence form. Physical DNA will be submitted shortly. | + | <p>Currently, this part is only present in sequence form. Physical DNA will be submitted shortly.</p> |
| - | < | + | <p>Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. <i>Analytical Biochemistry</i> <b>366</b>: 131-136 </p> |
| - | Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. <i>Analytical Biochemistry</i> <b>366</b>: 131-136 </p> | + | |
<br> | <br> | ||
Revision as of 12:26, 22 September 2010
BioBricks: Genomic BRIDGEs
For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!
BioBricks: Bacterial BRIDGEs
Biobrick #1
A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.
Currently, this part is only present in sequence form. Physical DNA will be submitted shortly.
Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366: 131-136
