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Revision as of 05:05, 17 September 2010 (view source) Jocelynatustc (Talk | contribs) (New page: Jocelyn: colony PCR to test pduN. colony PCR before miniprep ×40 double digest of ABJKTUV in promoter and pduN. storage of bacterial ×36) ← Older edit		Revision as of 09:37, 22 September 2010 (view source) Hildegard (Talk | contribs) Newer edit →
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	storage of bacterial ×36		storage of bacterial ×36
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		+	----
		+	Hilde:
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		+	Fastdigest cutting: pduABJKNTUV(E,X)
		+
		+	Ligation of Promotor-pduABJKNTUV
		+
		+	Transformation of ligation produce
		+
		+	Coating of Promotor-pduABJKNTUV onto Cl+ plate

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Revision as of 09:37, 22 September 2010

Jocelyn:

colony PCR to test pduN.

colony PCR before miniprep ×40

double digest of ABJKTUV in promoter and pduN.

storage of bacterial ×36

---

Hilde:

Fastdigest cutting: pduABJKNTUV(E,X)

Ligation of Promotor-pduABJKNTUV

Transformation of ligation produce

Coating of Promotor-pduABJKNTUV onto Cl+ plate