From 2010.igem.org
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Latest revision as of 19:48, 24 October 2010
Week 10
13-09-2010
Cloning
- Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).
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Other
- Competence of E.Coli DB3.1
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Movie
- Continued working on movie.
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14-09-2010
Cloning
- Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.
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Other
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15-09-2010
Cloning
- Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!
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Other
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16-09-2010
17-09-2010
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