Team:EPF Lausanne/Notebook/week10

From 2010.igem.org

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(Cloning)
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== Cloning ==
== Cloning ==
    
    
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*Western Blot with Immunotoxin, p25, 028, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice once with the Maerkl lab antibodies and once with Onya's antibodies.  
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*Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.  
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Revision as of 09:16, 15 September 2010









Contents

Week 10

13-09-2010

Cloning

  • Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Other

  • Competence of E.Coli DB3.1


Movie

  • Continued working on movie.

14-09-2010

Cloning

  • Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.

Other

15-09-2010

Cloning

Other

16-09-2010

Cloning

Other

17-09-2010

Cloning

Other