Team:UNIPV-Pavia/Calendar/September/settimana3

(Difference between revisions)

Revision as of 19:38, 13 September 2010

SEPTEMBER: WEEK 3

Unfortunately we discovered we tried to ligate I32 (E-S) to I37 (X-P) instead of I37 (E-X) ;-(

So this step will be repeated.

Trasformation of ligations

into E. coli DH5-alpha.

Inoculum of

into 5 ml LB+Amp for ligations of the following day.

Miniprep and quanfification of:

3-hours digestion:

Gel run/cut of samples

Gel run/cut for I26, I31 and I34.

and gel extraction:

We already had digested DNA so we could perform new ON ligations

and repeat

@@ Line 66: / Line 66: @@
 *I34: ng/ul
 -hours digestion:
-*I26: XbaI-PstI
+*I26: XbaI-PstI (Insert)
-*I31: EcoRI-SpeI
+*I31: EcoRI-SpeI (Insert)
-*I34: E-X
+*I34: E-X (Vector)
 Gel run/cut of samples
 [[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2setp_final.jpg|thumb|200px|center| Gel run/cut for I26, I31 and I34.]]