Team:EPF Lausanne/Notebook/week10

From 2010.igem.org

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== Cloning ==
== Cloning ==
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*Western Blot from yesterday finished. Observed one band which did NOT correspond to the right size. Furthermore the negative control failed! Reason not known. Maybe it is another protein with a His-tag which is present on the C3 plasmid.
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== Drosophila ==
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== Other ==
== Other ==
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*Gwen made us a chocolate cake!! Yeah!
 
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= 15-09-2010 =
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== Cloning ==
== Cloning ==
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*Western Blot from yesterday repeated. Same result as yesterday.
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== Drosophila ==
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== Other ==
== Other ==
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*Solange has a hangover! ;)
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== Movie ==
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== Other ==
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*Started making the stop motion movie.
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== Movie ==
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== Other ==
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*Continued working on movie.
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Revision as of 11:57, 13 September 2010









Contents

Week 10

13-09-2010

Cloning

  • Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Other

  • Competence of E.Coli DB3.1


Movie

  • Continued working on movie.

14-09-2010

Cloning

Other

15-09-2010

Cloning

Other

16-09-2010

Cloning

Other

17-09-2010

Cloning

Other