Team:EPF Lausanne/Notebook/week10

From 2010.igem.org

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== Cloning ==
== Cloning ==
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*Western Blot started of C3-S-Immunotoxin to check if the Immunotoxin is expressed.
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*Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).  
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== Drosophila ==
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== Other ==
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*Competence of E.Coli DB3.1
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== Movie ==
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*Continued working on movie.
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Revision as of 11:32, 13 September 2010









Contents

Week 10

13-09-2010

Cloning

  • Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Other

  • Competence of E.Coli DB3.1


Movie

  • Continued working on movie.

14-09-2010

Cloning

  • Western Blot from yesterday finished. Observed one band which did NOT correspond to the right size. Furthermore the negative control failed! Reason not known. Maybe it is another protein with a His-tag which is present on the C3 plasmid.

Drosophila

Other

  • Gwen made us a chocolate cake!! Yeah!

15-09-2010

Cloning

  • Western Blot from yesterday repeated. Same result as yesterday.

Drosophila

Other

  • Solange has a hangover! ;)

16-09-2010

Cloning

Movie

  • Started making the stop motion movie.

17-09-2010

Cloning

Movie

  • Continued working on movie.









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