"

From 2010.igem.org

Revision as of 18:39, 12 September 2010 (view source) Ebrinkman (Talk | contribs) (New page: =Protein content measurements (Bradford assay)= ''Materials:'' - 2 mg/mL BSA - bradford reagens - spectrophotometer ''Protocol:'' 1. Make a dilution serie of standard 2 mg/mL bovine ...) ← Older edit		Latest revision as of 18:39, 12 September 2010 (view source) Ebrinkman (Talk | contribs)
Line 8:		Line 8:
	- spectrophotometer		- spectrophotometer
		+
	''Protocol:''		''Protocol:''

---

Latest revision as of 18:39, 12 September 2010

Protein content measurements (Bradford assay)

Materials:

- 2 mg/mL BSA

- bradford reagens

- spectrophotometer

Protocol:

1. Make a dilution serie of standard 2 mg/mL bovine serum albumin (BSA). Use 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 μL

2. Add MillQ to a total volume of 800 μL

3. Add 200 μL Bradford reagens and mix

4. Measure after minimal 5 min and max 60 min the OD595