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From 2010.igem.org

Protein content measurements (Bradford assay)

Materials:

- 2 mg/mL BSA

- bradford reagens

- spectrophotometer

Protocol:

1. Make a dilution serie of standard 2 mg/mL bovine serum albumin (BSA). Use 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 μL

2. Add MillQ to a total volume of 800 μL

3. Add 200 μL Bradford reagens and mix

4. Measure after minimal 5 min and max 60 min the OD595