Team:SDU-Denmark/labnotes9

From 2010.igem.org

(Difference between revisions)
(Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3)
(Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3)
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== Flagella Group ==
== Flagella Group ==
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=== Restriction Digest of Gel Extracted FlhDCmut and pSB1C3 and pSB1AK3 ===
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=== Restriction Digest of Gel Extracted FlhDCmut, pSB1C3 and pSB1AK3 ===
'''Date:''' 9/12 2010<Br>
'''Date:''' 9/12 2010<Br>
'''Done By:''' Sheila<Br>
'''Done By:''' Sheila<Br>
'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br>
'''Protocol:''' [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]<Br>
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'''Notes:''' <Br>
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'''Notes:''' The restriction digests are made in preperation for two different ligations, the [https://2010.igem.org/Team:SDU-Denmark/labnotes9#Ligation_of_FlhDCmut_and_pSB1C3 first] is the ligation of FlhDCmut into the submission backbone; pSB1C3. The [https://2010.igem.org/Team:SDU-Denmark/labnotes9#Ligation_of_FlhDCmut_and_pSB1AK3 second] is the the ligation of FlhDCmut into pSB1AK3. Different restriction enzymes were used in, as shown in the following tables<br><br>
''Restriction Digest mixtures''<Br>
''Restriction Digest mixtures''<Br>
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Following digestion, the samples were loaded and run on a 1.5% agarose gel, before being extracted prior to ligation.
'''Results:''' <Br>
'''Results:''' <Br>
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Revision as of 18:08, 12 September 2010