Team:USTC/Notebook/Meetings/10 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: Jocelyn: ligation of NTUV as insert into ABJK in vector PSB1C3.=>transformation into TOP 10.=>select single colony from plate (15 in all)=>colony PCR test select single colony from plat...) |
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select single colony from plate of pduAB (8 each ) pduNU (6 each) pduUV (6 each)in new standard. | select single colony from plate of pduAB (8 each ) pduNU (6 each) pduUV (6 each)in new standard. | ||
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+ | ---- | ||
+ | Hilde: | ||
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+ | Transformation : ligation product of pduABJK-pduNTUV into Trans 10 | ||
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+ | miniprep: plm.NTUV |
Latest revision as of 08:46, 22 September 2010
Jocelyn:
ligation of NTUV as insert into ABJK in vector PSB1C3.=>transformation into TOP 10.=>select single colony from plate (15 in all)=>colony PCR test
select single colony from plate of pduAB (8 each ) pduNU (6 each) pduUV (6 each)in new standard.
Hilde:
Transformation : ligation product of pduABJK-pduNTUV into Trans 10
miniprep: plm.NTUV