"

From 2010.igem.org

Jocelyn:

ligation of NTUV as insert into ABJK in vector PSB1C3.=>transformation into TOP 10.=>select single colony from plate (15 in all)=>colony PCR test

select single colony from plate of pduAB (8 each ) pduNU (6 each) pduUV (6 each)in new standard.

---

Hilde:

Transformation : ligation product of pduABJK-pduNTUV into Trans 10

miniprep: plm.NTUV