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Team:TU Delft/Project/rbs-characterization/parts
From 2010.igem.org
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BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations. | BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations. | ||
Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis. | Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis. | ||
Revision as of 16:39, 27 September 2010
BioBricks, the making of
BBa_I13401 was PCR amplified using the universal primers G00100 and G00101. The purified PCR product was assembled into one of the Anderson RBS plasmids provided in the distribution plates by means of 2-way ligations.
Transformation into competent Top10 E.coli cells yielded positives, as determined by fluorescence analysis.
