Team:Newcastle/10 September 2010

From 2010.igem.org

(Difference between revisions)
(Arabinose-inducible promoter characterisation)
(yneA)
Line 3: Line 3:
=''yne''A=
=''yne''A=
-
Nanodrop results:
+
Nanodrop results for pSB1C3 + ''yne''A:
#158.7
#158.7

Revision as of 13:40, 10 September 2010

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Contents

yneA

Nanodrop results for pSB1C3 + yneA:

  1. 158.7
  2. 261.2
  3. 262.9
  4. 204.7
  5. 262.9
  6. 202.1
  7. 174.8
  8. 226.6
  9. 229.1
  10. 164.5
  11. 177.8
  12. 225.1
  13. 281.5
  14. 249.3
  15. 192.2
  16. 239.3
  17. 195.1

Arabinose-inducible promoter characterisation

...

Miniprep of BBa_I13401 (GFP coding sequence + terminator)

..from last night's overnight cultures from the 8th of september transformation.

Double digests

.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..

Gel extraction of digests

Ligation

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