Team:KAIST-Korea/Notebook/Diary/September
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+ | <td><b>Materials</b></td><td><b>Volume</b></td> | ||
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+ | <td>STAT1(KRIBB) (template)</td><td></td> | ||
+ | </tr> | ||
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+ | <td>V_STAT_F</td><td></td> | ||
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+ | <td>V_STAT_R</td><td></td> | ||
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+ | <td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td></td> | ||
+ | </tr> | ||
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+ | <td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td></td> | ||
+ | </tr> | ||
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+ | <td>10x buffer (10x cloned Pfu Reaction buffer)</td><td></td> | ||
+ | </tr> | ||
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+ | <td>DW (3rd sterile water)</td><td></td> | ||
+ | </tr> | ||
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+ | <td>Total</td><td>50ul</td> | ||
+ | </tr> | ||
+ | </table> | ||
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[[Image:PCR_cycle.jpg]] | [[Image:PCR_cycle.jpg]] | ||
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[[Image:PCR_FGFR.jpg]] | [[Image:PCR_FGFR.jpg]] | ||
[[Image:PCR_HR.jpg]] | [[Image:PCR_HR.jpg]] | ||
[[Image:PCR_GAS.jpg]] | [[Image:PCR_GAS.jpg]] | ||
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+ | == September next == | ||
Revision as of 13:23, 10 September 2010
September 1pREP41 vector has arrived. -> we will do E.coli transformation and increase its number. pREP42-GFP vector has arrived (in E.coli) -> will incubate a day and do spreading for further experiment. Making culture media
Culture
September ?<STAT1> V_STAT1 (expression)
September next |