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Team:Tsinghua/Notebook/31 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == YX's part == PCR amplify the resistance genes. (First round) Then ligate the PCR products into T vector (pEASY T1, TransGen) After transformation, the trans1-T1 cells were spread onto...) |
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== YX's part == | == YX's part == | ||
| - | + | Picked three clones of each resistance gene and shook in 3ml LB media. | |
| - | + | Extracted the plasmid and did PCR to verify the sequence. | |
| - | + | The positive clones were sent for sequencing. | |
Revision as of 14:54, 9 September 2010
YX's part
Picked three clones of each resistance gene and shook in 3ml LB media.
Extracted the plasmid and did PCR to verify the sequence.
The positive clones were sent for sequencing.
