Team:UNIPV-Pavia/Calendar/September/settimana2

From 2010.igem.org

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(September, 6th)
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Glycerol stocks of MG43, MC42, MC43. Static TECAN test (absorbance, green fluorescence and red fluorescence) on 200 ul of these cultures.   
Glycerol stocks of MG43, MC42, MC43. Static TECAN test (absorbance, green fluorescence and red fluorescence) on 200 ul of these cultures.   

Revision as of 11:29, 9 September 2010


SEPTEMBER: WEEK 2



September, 6th

Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.


  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
  • Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
  • Inoculum for MiniPrep and sequencing of:
    • <partinfo>BBa_J23100</partinfo>_4C5
    • <partinfo>BBa_J23101</partinfo>_4C5
    • <partinfo>BBa_J23105</partinfo>_4C5
    • <partinfo>BBa_J23106</partinfo>_4C5
    • <partinfo>BBa_J23110</partinfo>_4C5
    • <partinfo>BBa_J23114</partinfo>_4C5
    • <partinfo>BBa_J23116</partinfo>_4C5
    • <partinfo>BBa_J23118</partinfo>_4C5
    • I7-A
    • I8-5-D

Inoculum of cultures for TECAN test on self-inducible promoters:

  • I14-1
  • I15-1
  • I16-1
  • I17-1
  • I18-1
  • I19-1
  • I14_4C5-2
  • I15_4C5-2
  • I16_4C5-1
  • I17_4C5-1
  • I18_4C5-1
  • I19_4C5-1
  • I6-3
  • I3-1
  • <partinfo>BBa_B0031</partinfo>

Glycerol stocks of MG43, MC42, MC43. Static TECAN test (absorbance, green fluorescence and red fluorescence) on 200 ul of these cultures.

September, 7th

MiniPrep for:

CultureQuantifications (ng/ul)
<partinfo>BBa_J23100</partinfo>_4C5 11,8
<partinfo>BBa_J23101</partinfo>_4C5 39,7
<partinfo>BBa_J23105</partinfo>_4C5 25,4
<partinfo>BBa_J23106</partinfo>_4C5 29,7
<partinfo>BBa_J23110</partinfo>_4C5 24,7
<partinfo>BBa_J23114</partinfo>_4C5 15,4
<partinfo>BBa_J23116</partinfo>_4C5 20,7
<partinfo>BBa_J23118</partinfo>_4C5 20,4
I7-A 113,6
I8-5-D 127,6
I44-2 (Cm 34) 63,9
I51-3 (Cm 34) 100,4
I44-2 (Cm 12,5) 68,1
I51-3 (Cm 12,5) 135,4
I47 74,8

Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.

September, 8th

Inoculum of:

  • I31
  • I32
  • I34
  • I35
  • I36
  • I37
  • I40
  • I41
  • I20

into 5 ml LB + Amp; already available:

  • I21(E-S)
  • I26(E-S)
  • I0(E-X)

for second step of ligation of phasins/intein



September, 9th

Minipreps were quantified as follows:

  • I31: ng/ul
  • I32: ng/ul
  • I34: ng/ul
  • I35: ng/ul
  • I36: ng/ul
  • I37: ng/ul
  • I40: ng/ul
  • I41: ng/ul
  • I20: ng/ul

September, 10th

September, 11th