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Team:UNIPV-Pavia/Calendar/September/settimana3
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==September, 13th== | ==September, 13th== | ||
| + | Unfortunately we discovered we tried to ligate I32 (E-S) to I37 (X-P) instead of I37 (E-X) ;-( | ||
| + | So this step will be repeated. | ||
| + | |||
| + | Trasformation of ligations | ||
| + | *I52 | ||
| + | *I54 | ||
| + | *I55 | ||
| + | *I56 | ||
| + | *I57 | ||
| + | *I58 | ||
| + | into ''E. coli'' DH5-alpha. | ||
| + | |||
| + | Inoculum of | ||
| + | *I26 | ||
| + | *I31 | ||
| + | *I34 | ||
| + | into 5 ml LB+Amp for ligations of the following day. | ||
| + | |||
| + | ---- | ||
| + | |||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Revision as of 18:41, 13 September 2010
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