Team:UNIPV-Pavia/Calendar/September/settimana2

(Difference between revisions)

Revision as of 08:25, 7 September 2010

SEPTEMBER: WEEK 2

Inoculum of I47, I48, I49 into 5 ml LB+Amp for TECAN experiment of the following day.

Inoculum of I44-2 and I51-3 into 5 ml LB+Cm34 to evaluate MiniPrep yields.
Inoculum of I44-2 and I51-3 into 5 ml LB+Cm12,5 to evaluate MiniPrep yields and for sequencing.
Inoculum for MiniPrep and sequencing of:
- <partinfo>BBa_J23100</partinfo>_4C5
- <partinfo>BBa_J23101</partinfo>_4C5
- <partinfo>BBa_J23105</partinfo>_4C5
- <partinfo>BBa_J23106</partinfo>_4C5
- <partinfo>BBa_J23110</partinfo>_4C5
- <partinfo>BBa_J23114</partinfo>_4C5
- <partinfo>BBa_J23116</partinfo>_4C5
- <partinfo>BBa_J23118</partinfo>_4C5
- I7-A
- I8-5-D

Inoculum of cultures for TECAN test on self-inducible promoters:

MiniPrep for:

Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.

@@ Line 76: / Line 76: @@
 ==September, 7th==
+MiniPrep for:
+{|
+|''' Culture'''||'''Quantifications (ng/ul)'''
+|-
+| <partinfo>BBa_J23100</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23101</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23105</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23106</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23110</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23114</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23116</partinfo>_4C5 ||
+|-
+| <partinfo>BBa_J23118</partinfo>_4C5 ||
+|-
+| I7-A ||
+|-
+| I8-5-D ||
+|-
+| I44-2 (Cm 34) ||
+|-
+| I51-3 (Cm 34) ||
+|-
+| I44-2 (Cm 12,5) ||
+|-
+| I51-3 (Cm 12,5) ||
+|}
+----
+Cultures for TECAN test were diluted in the morning (h10:20) 1:100 (40ul in 4ml) and let grown at 37°C 220 rpm till they reach the desired OD.
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