Team:Kyoto/Parts

From 2010.igem.org

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(BioBrick)
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|<partinfo>J06702</partinfo>||mCherry, bacterial with RBS and forward terminator||2-8-E||<partinfo>pSB1A2</partinfo>||Ampicillin||869||2079||
|<partinfo>J06702</partinfo>||mCherry, bacterial with RBS and forward terminator||2-8-E||<partinfo>pSB1A2</partinfo>||Ampicillin||869||2079||
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|<partinfo>pSB3K3</partinfo>||RFP Coding Device||1-5-E||<partinfo>pSB3K3</partinfo>||Kanamycin||1069||2750||<partinfo>J04450</partinfo>(RFP) is iserted.
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|<partinfo>pSB3K3</partinfo>||RFP Coding Device||1-5-E||<partinfo>pSB3K3</partinfo>||Kanamycin||1069||2750||<partinfo>J04450</partinfo>(RFP) is inserted.
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Revision as of 14:48, 31 August 2010

Contents

BioBrick

NameDescriptionWell*1PlasmidResistanceInsert LengthVector LengthComment
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948RPU=1.4
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948RPU=0.3
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948RPU=0.7
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419<partinfo>J04450</partinfo>(RFP) is inserted.
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072For shipping parts.
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079For measurement of RPU.
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750<partinfo>J23101</partinfo>+<partinfo>E0240</partinfo>
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750<partinfo>J04450</partinfo>(RFP) is inserted.
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Original

NameDescriptionPlasmidResistanceInsert LengthVector LengthComment
K358000Lysis Cassette, S-R-Rz/Rz1 Sam7 ver.11300
K358002Lysis Cassette, S-R-Rz/Rz1 Sam7 ver.21300
K358004Lysis Cassette, S-R-Rz/Rz11300
K358006S Sam7370
K358008S370
K358010SΔTMD1 Sam7300
K358012SΔTMD1300