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Team:DTU-Denmark/Wet lab
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!align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span]] | !align="center" width="14%" style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: white;"|[[Team:DTU-Denmark/Gallery|<span style="color:white;">Gallery</span]] | ||
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| + | ===== Anti-Terminator experimental setup ===== | ||
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| + | === N-protein plasmid === | ||
| + | The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. [[#References]] | ||
| + | |||
| + | === References === | ||
| + | * NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989 | ||
Revision as of 11:47, 7 September 2010
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Anti-Terminator experimental setup
N-protein plasmid
The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References
References
- NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989
