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Team:Newcastle/25 August 2010
From 2010.igem.org
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We use the digested products from the six tubes that worked for ligation. | We use the digested products from the six tubes that worked for ligation. | ||
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| + | ==Transformation of ''B. Subtilis''== | ||
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| + | ===Aim=== | ||
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| + | To transform ''yneA'' into competent ''B. Subtilis''. | ||
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| + | ===Materials and Protocol=== | ||
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| + | Please refer to [[Team:Newcastle/Transformation_of_B._subtilis|transformation of ''B. subtilis'']]. | ||
=''rocF'' and Subtilin immunity= | =''rocF'' and Subtilin immunity= | ||
Revision as of 09:39, 26 August 2010
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Contents |
yneA
Gel Electrophoresis
Aim
To check if the digestion from yesterday worked.
Materials and Protocol
Please refer to gel electrophoresis for protocol.
Results
The result from gel electrophoresis:
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Figure 1 shows the double digest of 12 tubes of pGFPrrnB and yneA.
- Lane 1: Vector only
- Lane 2: Tube 1
- Lane 3: Tube 2
- Lane 4: Tube 3
- Lane 5: Tube 4
- Lane 6: Tube 5
- Lane 7: Tube 6
- Lane 8: Tube 7
- Lane 9: Tube 8
- Lane 10: Tube 9
- Lane 11: Tube 10
- Lane 12: Tube 11
- Lane 13: Tube 12
Discussion
The bands we got from the gel shows that digestion in tubes 1, 2, 4, 10, 11 and 12 worked.
Conclusion
We use the digested products from the six tubes that worked for ligation.
Transformation of B. Subtilis
Aim
To transform yneA into competent B. Subtilis.
Materials and Protocol
Please refer to transformation of B. subtilis.
rocF and Subtilin immunity
Gel extraction
PCR
   
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