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Team:Newcastle/25 August 2010
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The result from gel electrophoresis: | The result from gel electrophoresis: | ||
[[Image:Newcastle 25-08-2010 – Gel 1 (Ethidium Bromide).png|500px|centre]] | [[Image:Newcastle 25-08-2010 – Gel 1 (Ethidium Bromide).png|500px|centre]] | ||
| - | '''Figure 1''' | + | '''Figure 1''' shows the double digest of 12 tubes of pGFPrrnB and ''yneA''. |
| - | *'''Lane 1''': | + | *'''Lane 1''': pGFPrrnB + ''yneA'' 1 |
| + | *'''Lane 2''': pGFPrrnB + ''yneA'' 2 | ||
| + | *'''Lane 3''': pGFPrrnB + ''yneA'' 3 | ||
| + | *'''Lane 4''': pGFPrrnB + ''yneA'' 4 | ||
| + | *'''Lane 5''': pGFPrrnB + ''yneA'' 5 | ||
| + | *'''Lane 6''': pGFPrrnB + ''yneA'' 6 | ||
| + | *'''Lane 7''': pGFPrrnB + ''yneA'' 7 | ||
| + | *'''Lane 8''': pGFPrrnB + ''yneA'' 8 | ||
| + | *'''Lane 9''': pGFPrrnB + ''yneA'' 9 | ||
| + | *'''Lane 10''': pGFPrrnB + ''yneA'' 10 | ||
| + | *'''Lane 11''': pGFPrrnB + ''yneA'' 11 | ||
| + | *'''Lane 12''': pGFPrrnB + ''yneA'' 12 | ||
===Discussion=== | ===Discussion=== | ||
Revision as of 09:28, 26 August 2010
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Contents |
yneA
Gel Electrophoresis
Aim
To check if the digestion from yesterday worked.
Materials and Protocol
Please refer to gel electrophoresis for protocol.
Results
The result from gel electrophoresis:
.png)
Figure 1 shows the double digest of 12 tubes of pGFPrrnB and yneA.
- Lane 1: pGFPrrnB + yneA 1
- Lane 2: pGFPrrnB + yneA 2
- Lane 3: pGFPrrnB + yneA 3
- Lane 4: pGFPrrnB + yneA 4
- Lane 5: pGFPrrnB + yneA 5
- Lane 6: pGFPrrnB + yneA 6
- Lane 7: pGFPrrnB + yneA 7
- Lane 8: pGFPrrnB + yneA 8
- Lane 9: pGFPrrnB + yneA 9
- Lane 10: pGFPrrnB + yneA 10
- Lane 11: pGFPrrnB + yneA 11
- Lane 12: pGFPrrnB + yneA 12
Discussion
The bands we got from the gel shows that digestion in tubes 1, 2, 4, 10, 11 and 12 worked.
Conclusion
We use the digestion from the six tubes that worked for ligation.
rocF and Subtilin immunity
Gel extraction
PCR
   
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