Team:Newcastle/23 August 2010

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[[Image:Newcastle Gel 1 - 23-08-2010.jpg|700px|centre]]
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'''Figure 1''': Gel electrophoresis of plasmid pSB1C3 linearized fragments which are amplified by 4 different PCR reactions running at 4 different melting temperatures. A 1 kb DNA ladder
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'''Figure 1''': Gel electrophoresis of the amplified linearized plasmid pSB1C3 fragments ran at 4 different melting temperatures, Tms, (50, 60, 65, 70°C). A 1 kb DNA ladder was used on either side of lanes.
* '''Lane 1''': pSB1C3 fragment amplified at 55°C
* '''Lane 1''': pSB1C3 fragment amplified at 55°C
* '''Lane 2''': pSB1C3 fragment amplified at 55°C
* '''Lane 2''': pSB1C3 fragment amplified at 55°C

Revision as of 10:51, 24 August 2010

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Contents

pSB1C3 plasmid gel electrophoresis

Aims

The aim of this experiment is to run gel electrophoresis for the extracted and linearized plasmid pSB1C3 fragment which were amplified at 4 different melting temperatures by 4 separate PCR reactions.

Materials and Protocol

Please refer to: Gel electrophoresis for gel electrophoresis protocol.

Result

Newcastle Gel 1 - 23-08-2010.jpg

Figure 1: Gel electrophoresis of the amplified linearized plasmid pSB1C3 fragments ran at 4 different melting temperatures, Tms, (50, 60, 65, 70°C). A 1 kb DNA ladder was used on either side of lanes.

  • Lane 1: pSB1C3 fragment amplified at 55°C
  • Lane 2: pSB1C3 fragment amplified at 55°C
  • Lane 3: pSB1C3 fragment amplified at 60°C
  • Lane 4: pSB1C3 fragment amplified at 60°C
  • Lane 5: pSB1C3 fragment amplified at 65°C
  • Lane 6: pSB1C3 fragment amplified at 65°C
  • Lane 7: pSB1C3 fragment amplified at 70°C
  • Lane 8: pSB1C3 fragment amplified at 70°C

Discussion

Conclusion

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