Biobrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the egnetic circuit of the biobrick.

AbrB gene maybe a suitable gene to repress sporulation by overexpressing it. Can also activate antimicrobial peptides like TasA. Immune the concrete from future attack by concrete destroying bacteria? Need to find what other genes that are involve in the pathway of AbrB.

Number of competence cells not an issue. Sin operone to control a lot of pathway.

A. FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis. B. Is spider silk able to resist the alkali conditions? C. Is it possible to produce spider silk in Bacillus?

Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system.

Harsh to apply on May, June period

Too early to book flights and hotels. May have other members joining.

Next tutorial to be on the week after

Media:Flowchart_overview-1-.ppt‎ A. Need to be more in depth. ie. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why. B. Another flow chart to show the different stituation and how the bacteria response to it.

To contact sponser next week

A. Foxit not working

1. To draft email to Colin and Chris and show it to instructors first(everyone) 2. To continue to develop flow chart(everyone) 3. Tutorial on Wiki

        1. Chair-Richard 
        2. Minutes-Racheal
        3. Computer-Jenetta