Team:Stockholm/18 August 2010

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==Andreas==
==Andreas==
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===Site-directed mutagenesis of SOD & yCCS===
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''Continued from 17/8''
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====Plasmid prep====
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''From 17/8 ON cultures''
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Elution volume: 70 &mu;l dH<sub>2</sub>O
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{|border="1" cellpadding="2" cellspacing="0" align="right"
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!colspan="3"|DNA concentrations
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|-
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!Sample
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!Conc [ng/&mu;l]
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!A<sub>260</sub>/A<sub>280</sub>
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|-
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|SC1&rarr;pSB1C3.m-SOD 1
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|107.9
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|1.84
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|-
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|SC2&rarr;pSB1C3.m-SOD 2
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|105.5
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|1.83
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|-
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|yC1&rarr;pSB1C3.m-yCCS 1
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|101.2
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|1.86
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|-
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|yC4&rarr;pSB1C3.m-yCCS 4
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|106.4
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|1.80
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|}

Revision as of 15:27, 18 August 2010

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Andreas

Site-directed mutagenesis of SOD & yCCS

Continued from 17/8

Plasmid prep

From 17/8 ON cultures

Elution volume: 70 μl dH2O

DNA concentrations
Sample Conc [ng/μl] A260/A280
SC1→pSB1C3.m-SOD 1 107.9 1.84
SC2→pSB1C3.m-SOD 2 105.5 1.83
yC1→pSB1C3.m-yCCS 1 101.2 1.86
yC4→pSB1C3.m-yCCS 4 106.4 1.80
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