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Team:LMU-Munich/Notebook/Apoptosis
From 2010.igem.org
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== 8-10-2010 == | == 8-10-2010 == | ||
| - | + | Transforming competent cells | |
| + | |||
| + | - eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin) | ||
| + | |||
| + | - TEV recogn N Degron SF3 = pDS7 (Ampicillin) | ||
| + | |||
| + | - TEV p14 recogn = 190-6 (Ampicillin) | ||
| + | |||
| + | -> Protocol: https://www.openwetware.org/wiki/Transforming_chemically_competent_cells_protocol | ||
| + | |||
| + | - We added 2 µl DNA | ||
| + | |||
| + | - We plated out 200 µl | ||
| + | |||
| + | |||
| + | Plasmid Isolation | ||
| + | - CMV-Promoter Biobrick: BBa_J52034 | ||
| + | -> Protocol: http://www.promega.com/protcards/9fb093/fb093.pdf | ||
| + | - Prepared overnight culture, measured concentration of DNA | ||
| + | -> Poor results -> thrown away | ||
== 8-11-2010 == | == 8-11-2010 == | ||
Revision as of 12:47, 13 August 2010
Some test text in bold
We created following tests:
Example of a table
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Transforming competent cells
- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin)
- TEV recogn N Degron SF3 = pDS7 (Ampicillin)
- TEV p14 recogn = 190-6 (Ampicillin)
-> Protocol: https://www.openwetware.org/wiki/Transforming_chemically_competent_cells_protocol
- We added 2 µl DNA
- We plated out 200 µl
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Apoptosis Notebook
Contents
8-02-2010
8-03-2010
8-04-2010
header 1
header 2
header 3
row 1, cell 1
row 1, cell 2
row 1, cell 3
row 2, cell 1
row 2, cell 2
row 2, cell 3
8-05-2010
8-06-2010
8-07-2010
8-08-2010
8-09-2010
8-10-2010
Plasmid Isolation
- CMV-Promoter Biobrick: BBa_J52034
-> Protocol: http://www.promega.com/protcards/9fb093/fb093.pdf
- Prepared overnight culture, measured concentration of DNA
-> Poor results -> thrown away
8-11-2010
8-12-2010
8-13-2010
8-14-2010
8-15-2010
8-16-2010
8-17-2010
8-18-2010
8-19-2010
8-20-2010
8-21-2010
8-22-2010
