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Team:Newcastle/9 July 2010
From 2010.igem.org
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==Protocol== | ==Protocol== | ||
| - | * [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] | + | * [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5α pSB1AT3 containing ''lacI'' insert. |
==Inference== | ==Inference== | ||
Revision as of 13:38, 11 August 2010
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9 July 2010
Contents |
LacI BioBrick Construction
Aims
- To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
Materials
- Competent E. coli (DH5alpha strain)
- Ligation of lacI into pSB1AT3
Protocol
- Transformation of E. coli DH5α pSB1AT3 containing lacI insert.
Inference
- Ecoli DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
   
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